Home LABORATORY OF GENE EXPRESSION OPTIMIZATION


[Head of Laboratory ] [Staff of Laboratory] [Main scientific interests] [Main achievements] [Research cooperation] [Programs in which the Laboratory is engaged] [Selected publications]



Head of Laboratory:

    Sergei V. Benevolensky, Ph.D.,
    tel.:  +7(495)-648-0912
    e-mail: benevol AT inbi.ras.ru 

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Staff of Laboratory:

    Abianova Alfia, Junior Scientist
    Vavilova Ekaterina, Scientist
    Zacepin Sergei, Senior Scientist, Ph.D.
    Kljachko Elena, Senior Scientist, Ph.D.
    Mechedov Petr, Engineer
    Morozkina Elena, Scientist, Ph.D.
    Surina Elisaveta, Junior Scientist
    Chulkin Andrei, Junior Scientist
    Jagudin Timur, Junior Scientist

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Main scientific interests:

         Homo- and heterologous gene expression in yeast. Analysis of genetic factors implicated in heterologous secretion efficiency. Construction of strains producing foreign proteins.
         Regulation of gene expression coding for secreted proteins in Penicillium canescens.
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Main achievements:

         Saccharomyces cerevisiae strain was constructed producing secreted correctly folded human mechano growth factor (hMGF). Proteinase was identified degrading hMGF in wild type strains.
         Production of humanized antibody fragments in yeast expression system was achieved.
         Genetic system responsible for catabolite repression in fungi Penicillium canescens is discovered.
         Genetic system of multiple endoxylanases in Penicillium canescens is described.
         Producer strain for recombinant laccase вrametes sp. is constructed.
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Research cooperation:
    Institute of bioorganic chemistry RAS, RU
    Institute of experimental cardiology, RU
    All-Russian Centre for molecular diagnostic and therapy, RU
    Institute of immunological engineering, RU
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Programs in which the Laboratory is engaged:
         The program of Federal Agency on Science and Innovations.
         IPP PROGRAM ЋProduction of humanized monoclonal antibodies against Bacillus anthracis and Yersinia pestis for the urgent prophylaxis and therapy of exposed individualsЛ
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Selected publications:
    1. Marchenko AN, Kozlov DG, Svirshchevskaya EV, Viskova NY, Benevolensky SV. The p1 Protein of the Yeast Transposon Ty1 Can Be Used for the Construction of Bi-Functional Virus-Like Particles. J. Mol. Microbiol. Biotechnol. 2003, v.5(2), 97-104.
    2. Alekseeva L., Nekrasov A., Marchenko A., Shevchenko M., Benevolenskii S., Sapozhnikov A., Kurup V.P., Svirshchevskaya E. Criptic B-cell epitope identification through informational analysis of protein sequences. Vaccine, 2007, v. 25, 2688-2697.
    3. E. R. Surina,, E. V. Morozkina, A. N. Marchenko, A. A. Antipin, O. V. Mitkevich, V. V. Kushnirov, M. D. Ter-Avanesyan, S. V. Benevolensky. Selection of DNA Aptamers Specifically Interactingwith the Fibrillar Form of the Yeast Sup35 Protein. Molecular Biology, 2009, Vol. 43, No. 4, pp. 626–631.
    4. Morozkina E.V., Marchenko A.N., Keruchenko J.S., Keruchenko I.D., Khotchenkov V.P., Popov V.O., Benevolensky S.V. Proteinase B Disruption Is Required for High Level Production of Human Mechano-Growth Factor in Saccharomyces cerevisiae. J. Mol. Microbiol. Biotechnol. 2010, v. 18:188-194.
    5. A. R. Abyanova, A. M. Chulkin, E. A. Vavilova, T. V. Fedorova, D. S. Loginov, O. V. Koroleva, S. V. Benevolensky. A Heterologous Production of the Trametes hirsuta Laccase in the Fungus Penicillium canescens. Applied Biochemistry and Microbiology, 2010, Vol. 46, No. 3, pp. 313–317
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Last review: 12 November, 2012
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