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Head of Laboratory:

    Prof. BORIS B. DZANTIEV,
    tel.:  +7(495)-954-28-04, 954-31-42
    e-mail: dzantiev AT inbi.ras.ru,  bdzan AT online.ru

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Staff:

     Zherdev A.V., Ph.D., leading researcher

     Eremin S.A., Prof., senior researcher

     Vengerov Yu.Yu., Prof., leading researcher

     Byzova N.A., senior researcher

     Omelchenko M.D., Ph.D., senior researcher

     Zajko V.V., Ph.D., researcher

     Hendrickson O.D., Ph.D., senior researcher

     Platonova T.A., Ph.D., senior researcher

     Kutvitsky V.A., engineer

     Berlina A.N., Ph.D., researcher

     Pridvorova S.M., junior researcher

     Tugolukov A.E., engineer

     Safenkova I.V., Ph.D., researcher

     Sotnikov D.V., junior researcher

     Chernikh V.S., engineer

     Smirnova N.I., researcher

     Taranova N.A., post-graduate student

     Provorny A.A., assistant

     Zvereva E.A., Ph.D., researcher

     Pankratova G.K., assistant

     Tugolukov A.A., assistant

     Urusov A.E., Ph.D., junior researcher

     Petrakova A.V., assistant

     
     

     Staroverova N.P., assistant

     

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Fields of interests:
       1. Studies of quantitative regulations of antigen-antibody interactions.
       2. Development of new immunochemical techniques for the detection of biologically active compounds.
       3. Development of registration tools for bio-and immunochemical analytical systems.
       4. Safety of food stuffs and nanotechnology production.

       The researches carrying out in the laboratory are focused on the influence of immunoreagents properties on kinetic and equilibrium parameters of the antigen-antibody reaction. Models of immune complexes formation are developed. Structural changes of protein antigens in the course of their interaction with antibodies are studied, including antibody-assisted refolding and modulation of catalytic activities of enzymes. Compounds being important for medical diagnostics, quality control of agricultural and food products, environmental monitoring are used as model antigens in the study of the immunochemical processes. Among the studied objects are pesticides, hormones, antibiotics, mycotoxins, drugs, surfactants, enzymes, immunoglobulins, bacterial cells, plant viruses. This row enables to determine common regularities and specific features of the immunochemical interactions. For the detection of these compounds novel assay formats and marker systems are proposed allowing to reach lower limits of detection and higher rapidity of the assays. Efficiency of the proposed novel techniques in the course of their practical application is characterized; traditional and new methods of analysis are compared. Technologies for scaled production of immunoanalytical test-systems are elaborated jointly with manufacturers.
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Main achievements:

    1. The influence of the surface density of antigenic determinants on the formation of mono- and bivalent complexes with antibodies and changes in the effective binding constants have been characterized.
    2. The dependences between the composition of hapten-carrier conjugates and the parameters of their interaction with antibodies have been studied.
    3. Experimental model of multivalent antigens with unilamellar liposomes and lipid-hapten conjugates have been developed, which allows to vary the composition of the antigen, the rate of lateral diffusion and clustering of antigenic determinants.
    4. The effect of antibody-assisted refolding of protein antigens has been described.
    5. Changes in properties of such enzymes as α-amylase, peroxidase, β-galactosidase that are caused by their interaction with antibodies have been characterized.
    6. Factors affecting the specificity of the immunochemical reaction have been identified on the model of the interaction between antibodies with pesticides (triazines, aryl- and sulfonylureas, pyrethroids) and their metabolites have been studied.
    7. The process of antibodies immobilization on colloidal gold nanoparticles has been studied. The relationships between the degree of filling of the nanoparticles surface and binding constant of the interaction between immobilized antibodies with multivalent antigens have been studied.
    8. Approaches of atomic force microscopy have been applied for quantitative characterization of single antigen-antibody interactions and degree of surface modification of nanoparticles.
    9. Poly-and monoclonal antibodies against fullerene have been obtained. Regularities of the recognition of carbon nanoparticles structures by antibodies have been characterized.
    10. New methods of rapid immunochemical analysis based on linear water-soluble polyelectrolytes have been developed.
    11. The techniques of multiparametric immunochromatographic assay using two-dimensional array of binding points have been developed, providing tenfold improvement of performance and productivity of the assays.
    12. Techniques for on-site express assays with visual and instrumental registration have been developed for medical diagnostics, environmental monitoring and control of food stuffs.
    13. Immunochromatographic test systems using colloidal gold nanoparticles and fluorescent quantum dots for the detection of pesticides, mycotoxins, drugs, plant viruses, pathogenic microorganisms have been developed. Production of immunochromatographic test-systems for the detection of drugs of abuse and antibiotics has been organized jointly with "Innovational Biotechnologies" Ltd. And "RED" Ltd. The investigations were awarded by 2010 Premium of the Government of the Russian Federation in the field of science and technology.
    14. In the framework of the Federal Target Program Infrastructure development of nanotechnology in Russia complex of techniques methods to assess biological effects and safety of technogenic nanoparticles and nanotechnology products has been developed and validated. localization of nanoparticles in animals at different exposure modes and the effect of nanoparticles on the biochemical parameters of the body have been examined. 34 methodical instructions and guidelines for nanobiosafety being prepared with the participation of the laboratory staff, have been approved by Russian Federal Consumer Rights Protection and Human Health Control Service (Rospotrebnadzor).

    Prospects for research:
    1. Elucidation of the influence of non-equilibrium regime of immunochemical interactions on the analytical characteristics of heterogeneous immunoassays.
    2. Characterization of the composition of immune complexes formed in various systems of the detection of low and high molecular weight antigens.
    3. Characterization of the spatial structure of antibodies against carbon nanoparticles and their immune complexes.
    4. Development of new systems for multiparametric detection of biologically active compounds, which combine high selectivity and rapidity, including ones with the use of fluorescent markers having different spectral characteristics.
    5. Creation of express diagnostic techniques for of socially significant diseases of humans and farm animals.
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Unique/rare research methods (based on own equipment), available in the laboratory:
       In the framework of basic and applied researches the laboratory realizes full cycle of the development of immunoanalytical techniques, beginning from obtaining of antibodies and finalizing by the creation of manufacturing technologies. The given cycle embraces both traditional and specific methods and approaches (including the developed in the laboratory ones).
    Techniques for fundamental studies:
    • mathematical modeling of the formation of immune complexes differing in composition and valency;
    • original algorithms for digital processing of images for quantitative characterization of the interactions on membrane carriers;
    • characterization of immune complexes composition by dynamic light scattering, field flow fractionation, transmission electron and atomic force microscopy;
    • quantitative characterization of immunochemical interactions with the use of the registration of surface plasmon resonance (biosensoric system Biacore), and atomic force spectroscopy.
    Methods for obtaining of specific reactants:
    • obtaining, stabilization and characterization of colloidal carriers for immunochromatography;
    • obtaining and characterization of the composition and reactivity of intermolecular conjugates bertween protein carriers and derived antigens;
    • conjugation of immunoreactants with water-soluble polymeric carriers;
    • obtaining of liposomal antigenic preparations.
    Immunoanalytical systems:
    • microplate and membrane express assays with polyelectrolyte-based separation;
    • quantitative membrane (immunofiltration and immunochromatographic) analysis with photo and fluorimetric detection.
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Inter-institute and international scientific relations:
    M.V. Lomonosov Moscow State University, Chairs of Chemical Enzymology, Polymer Science, Analytical Chemistry & Virology
    I.M. Sechenov Moscow Medical Academy
    M.V. Lomonosov Moscow State Academy of Fine Chemical Technology
    Moscow State University of Food Production
    Kazan State University, Chair of Analytical Chemistry
    Lipetsk State Technical University
    M.M. Shemyakin & Yu.A. Ovchinnikov Institute of Bioorganic Chemistry Russian Acad. Sci., Moscow
    Institute of Physiologically Active Substances Russian Acad. Sci., Chernogolovka
    G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms Russian Acad. Sci., Pushchino
    Institute for Analytical Instrumentation Russian Acad. Sci., Sankt-Petersburg
    Institute of Biochemistry and Physiology of Plants and Microorganisms Russian Acad. Sci., Saratov
    Research Institute of Nutrition Russian Acad. Medical Sci., Moscow
    I.I. Mechnikov Research Institute for Vaccines and Sera Russian Academy of Medical Sciences, Moscow
    State Scientific Center Institute of Immunology, Moscow
    State Research Institute of Biological Instrumentation, Moscow
    Dairy Research Institute Russian Acad. Agricultural Sci., Moscow
    State Scientific Center of Applied Microbiology and Biotechnology, Obolensk
    Federal State Unitary Enterprise "All-Russian Research Institute of Metrological Service", Moscow
    A.V. Palladin Institute of Biochemistry, Kiyiv, Ukraine
    F.D. Ovcharenko Institute of Biocolloidal Chemistry, Kiyiv, Ukraine
    Institute of Bioorganic Chemistry, Minsk, Belarus
    National Center for Biotechnology, Astana, Kazakhstan
    RIKILT Institute of Food Safety, Wageningen, the Netherlands
    Institute of Sciences of Food Production, Bari, Italy
    University of Parma, Italy
    Institute of Occupational Medicine, Edinburgh, UK
    Tel-Aviv University, Israel
    Program on mycotoxins and experimental carcinogenesis, Cape Town, South Africa
    Institute of microbial technology, Chandigarh, India
    Institute of Technology, Varanasi, India
    Shanghai Jiatong University, Shanghai, China
    South China Agricultural University, Guangzhou, China
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Participation in grants and programs (2010-2012):
    Russian Foundation for Basic Research
    Programs of Fundamental Studies of the Presidium of the Russian Acad. Sci.:
        Development and improvement of methods of chemical analysis and study of the structure of substances and materials
        Fundamentals of technology of nanostructures and nanomaterials
    Federal Target Programs:
        Research and developments on priority directions of scientific-technological complex of Russia for 2007-2013
        Infrastructure development of nanotechnology in Russia for 2008-2011
        Scientific and scientific-pedagogical personnel of innovative Russia for 2009-2013
    International target program EurAsEC Innovative Biotechnologies
    Projects of the Seventh Framework Programme of the European Community:
        Novel integrated strategies for worldwide mycotoxin reduction in the food and feed chains (MYCORED)
        Managing risks of nanomaterials (MARINA)
        Towards sustainable global food safety collaboration (Collab4Safety)
    Long-term program of the Russian-Indian scientific and technological cooperation
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Selected recent publications:
    1. Byzova N.A., Safenkova I.V., Chirkov S.N., Avdienko V.G., Guseva A.N.., Mitrofanova I.V., Zherdev A.V., Dzantiev B.B., Atabekov J.G. Interaction of plum pox virus with specific colloidal gold-labeled antibodies and development of immunochromatographic assay of the virus. Biochemistry (Moscow). 2010, v. 75, No 11, pp. 1393-1403.
    2. Byzova N.A., Zherdev A.V., Dzantiev B.B. Immunochromatographic assay with photometric detection for rapid determination of atrazine and other triazine herbicides in foodstuffs. Journal of AOAC International. 2010, v. 93, No 1, pp. 36-43.
    3. Byzova N.A., Zvereva E.A., Zherdev A.V., Eremin S.A., Dzantiev B.B. Rapid pretreatment-free immunochromatographic assay of chloramphenicol in milk. Talanta. 2010, v. 81, No 3, pp. 843-848.
    4. Safenkova I.V., Zherdev A.V., Dzantiev B.B. Correlation between the composition of multivalent antibody conjugates with colloidal gold nanoparticles and their affinity. Journal of Immunological Methods. 2010, v. 357, No 12, pp. 1725.
    5. Sakharov I.Yu., Berlina A.N., Zherdev A.V., Dzantiev B.B. Advantages of soybean peroxidase over horseradish peroxidase as the enzyme label in chemiluminescent ELISA of sulfamethoxypyridazine. Journal of Agricultural and Food Chemistry. 2010, v. 58, No 6, pp. 3284-3288.
    6. Tafintseva I.Yu., Zherdev A.V., Eremin S.A., Dzantiev B.B. Enzyme immunoassay for determination of sulfamethoxypyridazine in honey. Applied Biochemistry and Microbiology. 2010, v. 46, No 2, pp. 216-220.
    7. Urusov A.E., Zherdev A.V., Dzantiev B.B. Immunochemical methods of mycotoxin analysis. Applied Biochemistry and Microbiology. 2010, v. 46, No 3, pp. 253-266.
    8. Bekasova O.D., Chebotarev N.A., Safenkova I.V., Rusanov A.L., Kurganov B.I. Effect of CdS nanoparticles on the properties of a protein matrix. Inorganic Materials. 2011, v. 47, No 8, pp. 830-836.
    9. Byzova N.A., Zvereva E.A., Zherdev A.V., Dzantiev B.B. Immunochromatographic technique for express determination of ampicillin in milk and dairy products. Applied Biochemistry and Microbiology. 2011, v. 47, No 6, pp. 627-634.
    10. Byzova N.A., Zvereva E.A., Zherdev A.V., Eremin S.A., Sveshnikov P.G., Dzantiev B.B. Pretreatment-free immunochromatographic assay for the detection of streptomycin and its application to the control of milk and dairy products. Analytica Chimica Acta. 2011, v. 701, No 2, pp. 209-217.
    11. Hendrickson O.D., Fedyunina N.S., Martianov A.A., Zherdev A.V., Dzantiev B.B. Production of anti-fullerene C60 polyclonal antibodies and study of their interaction with a conjugated form of fullerene. Journal of Nanoparticle Research. 2011, v. 13, No 9, pp. 3713-3719.
    12. Hendrickson O.D., Safenkova I.V., Zherdev A.V., Dzantiev B.B., Popov V.O. Methods of detection and identification of manufactured nanoparticles. Biophysics. 2011, v. 56, No 6, pp. 961-986.
    13. Panchenko L.F., Starovoitova T.A., Vengerov Yu.Yu., Dzantiev B.B., Davydov B.V. [Analytical video digital complexes for express and screening laboratory studies in drug control.] Narkologiya. 2011, No 12, pp. 64-68. (In Russian.)
    14. Urusov A.E., Kostenko S.N., Sveshnikov P.G., Zherdev A.V., Dzantiev B.B. Immunochromatographic assay for the detection of ochratoxin A. Journal of Analytical Chemistry. 2011, v. 66, No 8, pp. 770-776.
    15. Urusov A.E., Kostenko S.N., Sveshnikov P.G., Zherdev A.V., Dzantiev B.B. Ochratoxin A immunoassay with surface plasmon resonance registration: Lowering limit of detection by the use of colloidal gold immunoconjugates. Sensors and Actuators B: Chemical. 2011, v. 156, No 1, pp. 343-349.
    16. Byzova N.A., Zherdev A.V., Eskendirova S.Z., Baltin K.K., Unysheva G.B., Mukanov K.K., Ramankulov E.M., Dzantiev B.B. Development of immunochromatographic test system for rapid detection of the lipopolysaccharide antigen and cells of the causative agent of bovine brucellosis. Applied Biochemistry and Microbiology. 2012, v. 48, No 6, pp. 590-597.
    17. Hendrickson O.D., Fedyunina N.S., Zherdev A.V., Solopova O.N., Sveshnikov P.G., Dzantiev B.B. Production of monoclonal antibodies against fullerene C60 and development of a fullerene enzyme immunoassay. Analyst. 2012, v. 137, No 1, pp. 98-105.
    18. Safenkova I.V., Zherdev A.V., Dzantiev B.B. Application of atomic force microscopy for characteristics of single intermolecular interactions. Biochemistry (Moscow). 2012, v. 77, No 13, pp. 1536-1552.
    19. Safenkova I.V., Zherdev A.V., Dzantiev B.B. Factors influencing the detection limit of the lateral-flow sandwich immunoassay: a case study with potato virus X. Analytical and Bioanalytical Chemistry. 2012, v. 403, No 6, pp. 1595-1605.
    20. Gmoshinski I.V., Khotimchenko S.A., Popov V.O., Dzantiev B.B., Zherdev A.V., Demin V.F., Buzulukov Yu G. Nanomaterials and nanotechnologies: methods of analysis and control. - Russian Chemical Reviews. 2013, v. 82, No 1, pp. 48-76.
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Last review: 18 January, 2013
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