Home LABORATORY BIOCHEMISTRY OF CHLOROPLAST


[ Head of Laboratory ] [ Staff of Laboratory] [ Field of Interests] [ Main achievements] [Collaboration] [Curren Grants] [Selected Publications]


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The Laboratory was organized in 1986

Head of Laboratory:

    Karapetyan Navassard Vaganovich, Professor, Dr. Sci. (Biology)
    Winner of the USSR State Prize on Science and Technology (1991)

    tel.:  +7(495)-952-1505
    e-mail: nkarap@inbi.ras.ru

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Staff of Laboratory:


    Yurina Nadezda Petrovna Dr.Sci., Prof.
    Odintsova Margarita Semenovna Dr.Sci., Prof.
    Shubin Vladimir Veniaminovich Ph.D.
    Bolychevtseva Yulia Vladimirovna Ph.D.
    Terekhova Irina Vladimirovna Ph.D.
    Oleskina Yulia Petrovna Ph.D.
    Rabinovich Mikhail Lvovich Dr.Sci., Prof.
    Vasilchenko Lilia Grigorievna Ph.D.
    Karapetyan Karen Navasardovich Ph.D.
    Ershevich Olga Petrovna
    Mokerova Daria Valerievna Ph.D. Student

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Field of Interests:

    Mechanisms of function of photosystem I and photoprotection in cyanobacteria:
    Characteristics of the antenna of photosystem 1 trimers and monomers; origin of the long-wavelength chlorophylls and their role in photosynthesis; the reason of high stability of P700 and the long-wavelength chlorophylls to high irradiance; the mechanisms of stress resistance of the photosynthetic apparatus of cyanobacteria; pathways of dissipation of excess absorbed energy in photosystem 1 trimers; dependence of energy migration within antenna of photosystem 1 trimers and monomers on redox state of the reaction center cofactors; mechanisms of non-photochemical quenching in cyanobacteria; energy migration from phycobilisomes to photosystem 1 and photosystem 2 in cyanobacteria; effect of interaction of the photosynthetic and respiratory chains on state transitions in cyanobacteria.
    Molecular Biology of Chloroplasts:
    Mechanisms of regulation of gene expression of plastid proteins; processes responsible for coordinated expression of nuclear and chloroplast genes that code of plastid proteins; study of the role of chloroplasts in expression of nuclear genes that code plastid proteins; structure-function study of genome of chloroplast and other plastids.

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Main achievements:

        It is shown the preferential organization of PSI complex in the membranes of cyanobacteria as trimers that are highly enriched with long-wavelength chlorophylls. The most long-wave chlorophyll with 77K emission band at 760 nm in PSI trimers of Arthrospira platensis is formed in result of interaction of chlorophylls localized on different monomeric complexes. When P700 is oxidized in one of monomeric complexes within a trimer, interaction of these long-wave chlorophylls stimulates energy dissipation thus protecting complex against photodestruction. Fluorescence yield of PSI trimers at 760 nm (77K) strongly dependes on the redox state of P700: maximum with P700 reduced and minimum with P700 oxidized. If the cofactors of the acceptor side of PSI are pre-reduced, P700 triplet (formed in result of recombination of P700+ and Ao-) quenches fluorescence at 760 nm. The picosecond rate of energy migration from chlorophylls to cation radical of P700 in PSI trimers of A. platensis is higher than that to P700 reduced. Long-wave chlorophylls delay the energy equilibration in antenna and trapping by P700. It is shown the highest photostability of p700 cation radical.
        New photoprorective pathway of energy dissipation in the pigment apparatus of Synechocystis sp. PCC 6803 (so called non-photochemical quenching) monitored as blue-green light induced fluorescence quenching is found. According to action spectrum of quenching, carotenoid serves as a photosensitizer of quenching of phycobilisomes fluorescence at 662 and ~680 nm; quenching is reversible in the dark. This quenching diverts the energy flow from phycobilisomes to both photosystems thus protecting the photosynthetic apparatus of cyanobacteria against photodestruction. Blue-green light induced quenching is observed even in PSI/PSII-less mutant strain of Synechocystis sp. PCC 6803. Quenching center formed by blue-green light is able to quench the phycobilisome fluorescence in the dark.
        It was proven that there is a chloroplast control of the gene transcription of light stress proteins ELIP1 and ELIP2 in Arabidopsis thaliana L. Plastid signals can differently regulate expression of genes of a closely related multigene family of photosynthesis proteins ELIP and Lhcb2. Expression of ELIP genes is influenced by signals of either exogenous (light) or endogenous origin (plastid signals, hormones, carbohydrates). These types of signals may tightly interact with each other and inhibit (or activate) ELIP1 and ELIP2 gene expression and, in this way, increase plant resistance to the action of stress factors of the environment. It is shown that overexpression of chloroplast heat shock protein HSP70B could be considered as an early biomarker of oxidative stress, and is one of the factors that determining thermotolerance green algae. It was reveled that processing and import of precursors of stress proteins HSP32 and ELIP into plastids are disturbed in norflurazon-treated seedlings. Accumulation of precursors of stress proteins in envelope of plastids are detected after the photodestruction of chloroplasts. The DNA molecules arranged with proteins into compact transcriptionally active structures the nucleoids were purified from chloroplasts of higher plants and algae. Composition of proteins of nucleoids is inconstant and depended on stage of differentiation of organelle. However in differentiated chloroplasts the composition of nucleoid proteins is conservative for different plant species.


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Collaboration:

    Max-Volmer-Laboratory of Biophysical Chemistry, Berlin Technical University, Germany (Dr. E. Schlodder)
    Department of Plant Biochemistry, Faculty of Biology and Biotechnology, Ruhr-University-Bochum, Germany (Prof. M. Rogner)
    Institute of Physical and Theoretical Chemistry, Tubingen University, Germany (Dr. M. Brecht)
    Department of Genetics, Biology Faculty, M.V. Lomonosov Moscow State University (Dr. I.V. Elanskaya)
    Institute of General Ecology, Bulgarian Academy of Sciences, Sofia, Bulgaria (Dr. S. Chankova).

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Participation in projects and programs:

    Program of Russian Academy of Sciences Molecular and Cell Biology, 2004-2008
    project Bioenergetics of Photosynthesis and Respiration under stress
    head Prof. N.V. Karapetyan
    Program of Russian Academy of Sciences Molecular and Cell Biology, 2009-2013
    project Molecular mechanisms of protection of the photosynthetic apparatus against intense irradiation
    head Prof. N.V. Karapetyan
    11 RFBR grants (Karapetyan N.V., Odintsova M.S.)
    3 NATO Scientific Affairs grants (Karapetyan N.V.)
    DFG grant (Yurina N.P.)
    INTAS grant (Odintsova M.S.)

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Selected Publications:

      Kruip J., Karapetyan N.V., Terekhova I.V., Rogner M. In vitro oligomerisation of a membrane protein complex: liposome based reconstitution of trimeric photosystem I from isolated monomers. J. Biol. Chem. (1999) 274, 18181-18188
      Witt H., Bordignon E., Carbonera D., Dekker J.P., Karapetyan N.V., Teutloff C., Webber A., Lubitz W., Schlodder E. Species-specific differences of the spectroscopic properties of P700: analysis of influence of non-conserved amino acid residues by site-directed mutagenesis of photosystem I from Chlamydomonas reinhardtii. J. Biol. Chem. (2003) 278, 46760-46771
      Cometta A., Zucchelli G., Karapetyan N.V., Engelmann E., Garlaschi F.M., Jennings R.C. Thermal bahavior of long wavelength absorption transitions in Spirulina platensis photosystem I trimers. Biophys. J. (2000) 79, 3235-3243
      Gobets B., van Stokkum I.H.M., Rogner M., Kruip J., Schlodder E., Karapetyan N.V., Dekker J.P., van Grondelle R. Time-resolved fluorescence emission measurements of photosystem I particles of various cyanobacteria: a unified compartmental model. Biophys. J. (2001) 81, 407-424
      Karapetyan N.V., Dorra D., Schweitzer G., Bezsmertnaya I.N., Holzwarth A.R. Fluorescence spectroscopy of the longwave chlorophylls in trimeric and monomeric photosystem I core complexes from cyanobacterium Spirulina platensis. Biochemistry. (1997) 36, 13830-13837
      Rakhimberdieva M.G., Boichenko V.A., Karapetyan N.V., Stadnichuk I.N. Interaction of phycobilisomees with photosystem 2 dimers and photosystem 1 monomers and trimers of the cyanobacterium Spirulina platensis. Biochemistry. (2001) 40, 15780-15788
      El-Mohsnawy E., Kopczak M.J., Schlodder E., Nowaczyk M., Meyer H.E., Warscheid B., Karapetyan N.V., Rogner M. (2010) Structure and function of intact photosystem 1 monomers from the cyanobacterium Thermosynechococcus elongatus. Biochemistry. 49, 4740-4751
      Frese R.N., Palacios M.A., Azzizi A., van Stokkum I.H.M., Kruip J., Rogner M., Karapetyan N.V., Schlodder E., van Grondelle R., Dekker J.P. Electric field effects on red chlorophylls, β-carotenes and P700 in cyanobacterial photosystem I complexes. Biochim. Biophys. Acta. (2002) 1554, 180-191
      Schlodder E., Cetin M., Byrdin M., Terekhova I.N., Karapetyan N.V. P700+- and 3P700-induced quenching of the fluorescence at 760 nm in trimeric photosytem I complexes from the cyanobacterium Arthrospira platensis . Biochim. Biophys. Acta. (2005) 1706, 53-67
      Schlodder E., Shubin V.V., El-Mohsnawy E., Rogner M., Karapetyan N.V. (2007) Steady-state and transient polarized absorption spectroscopy of photosytem I complexes from the cyanobacteria Arthrospira platensis and Thermosynechococcus elongatus . Biochim. Biophys. Acta. 1767, 732-741
      Rakhimberdieva M.G., Vavilin D.V., Vermaas W.F.J., Elanskaya I.V., Karapetyan N.V. (2007) Phycobilin/chlorophyll excitation equilibration upon carotenoid-induced non-photochemical fluorescence quenching in phycobilisomes of the cyanobacterium Synechocystis sp. PCC 6803. Biochim. Biophys. Acta. 1767, 757-765
      Rakhimberdieva M.G., Elanskaya I.V., Vermaas W.F.J., Karapetyan N.V. (2010) Carotenoid-triggered energy dissipation in Synechocystis phycobilisomes diverts excitation away from reaction centers of both photosystems. Biochim. Biophys. Acta. 1797, 241-249
      Schlodder E., Hussels M., Karapetyan N.V., Brecht M. (2011) Fluorescence of the various red antenna states in photosytem I complexes from cyanobacteria is affected differently by the redox state of P700. Biochim. Biophys. Acta. 1807, 1423-1431
      Brecht M., Hussels M., Schlodder E., Karapetyan N.V. (2012) Red antenna states of photosystem I trimers from Arthrospira platensis revealed by single-molecule spectroscopy. Biochim. Biophys. Acta. 1817, 445-452
      Kuzminov F.I., Karapetyan N.V., Rakhimberdieva M.G., Elanskaya I.V., Gorbunov M.Y., Fadeev V.V. (2012) Investigation of OCP-triggered dissipation of excitation energy in PSI/PSII-less Synechocystis sp. PCC 6803 mutant using non-linear laser fluorimetry. Biochim. Biophys. Acta. 1817, 1012-1021
      Karapetyan N.V., Holzwarth A.R., Rogner M. The photosystem I of cyanobacteria: molecular organization, excitation dynamics, and physiological significance. FEBS Lett. (1999) 460, 395-400
      Rakhimberdieva M.G., Stadnichuk I.N., Elanskaya I.V., Karapetyan N.V. Carotenoid-induced quenching of the phycobilisome fluorescence in photosystem II-deficient mutant of Synechocistis sp. FEBS Lett. (2004) 574, 85-88
      Rakhimberdieva M.G., Bolychevtseva Y.V., Elanskaya I.V., Karapetyan N.V. (2007) Protein-protein interactions in carotenoid triggered quenching of phycobilisome fluorescence in Synechocystis sp. PCC 6803. FEBS Lett. 581, 2429-2433
      Rakhimberdieva M.G., Kuzminov F.I., Elanskaya I.V., Karapetyan N.V. (2011) Synechocystis sp. PCC 6803 mutant lacking both photosystems exhibits strong carotenoid-induced quenching of phycobilisome fluorescence. FEBS Lett. 585, 585-589
      Shubin VV., Terekhova I.N., Kirillov B.A., Karapetyan N.V. (2008) Quantum yield of P700+ photodestruction in isolated photosystem I complexes of the cyanobacterium Arthrospira platensis . Photochem. Photobiol. Sci. 7, 956-962
      Karapetyan (2008) Protective dissipation of excess absorbed energy by photosynthetic apparatus of cyanobacteria: role of antenna terminal emitters. Photosynth. Res. 97, 195-204
      Karapetyan N.V., Schlodder E., van Grondelle R., Dekker J.P. (2006) Long wavelength chlorophylls of photosystem I. In Photosystem I: The Light-Driven, Plastocyanin/Ferredoxin/Oxydoreductase (Golbeck J.H., ed.) in series . Advances in Photosynthesis and Respiration. , Springer, vol. 24, pp. 177-192
      .. (2007) . . 72, 1385-1395
      Yurina N.P., Kloppstech K. Accumulation of plastid protein precursors under norflurazon-induced carotenoid deficiency and oxidative stress in barley. Plant Physiol. Biochem. (2001) 39, 807-814

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Last review: 24, October, 2012
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