Home LABORATORY OF ENZYME SYSTEMS


[Head of Laboratory ] [Area of Interests] [The Most Significant Achievements] [Unique Equipment and/or Methods] [Partners] [Grants ] [Selected publications] [COMPUTER BIOCHEMISTRY GROUP]


LABORATORY OF ENZYME SYSTEMS



The Laboratory was organized in 1990

Head of Laboratory:

    Kurganov Boris Ivanovich, D. Sci., Professor, USSR State Prize, Honoured Scientist of Russian Federation

    tel.:  +7(495)-952-5641
    e-mail: kurganov AT inbi.ras.ru

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Area of scientific interests, priorities:
    Protein stability, protein aggregation, chaperones, nanobiotechnology.
Main directions of the present activity:
    The study of the mechanisms of protein aggregation and the mechanisms of the protective action of natural and artificial chaperones. The study of interaction of nanoparticles with proteins. Development of protein nanoparticles for biotechnological aims.
The most successful projects:
    “Stability, denaturation, aggregation, and refolding of oligomeric enzymes”.
The present project:
    "Protein aggregation. Mechanisms of the protective action of molecular chaperones" (the project is fulfilled in collaboration with Laboratory of molecular organization of biological structures).
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The most significant achievements:
    The new mechanism of protein aggregation leading to formation of amorphous aggregates has been experimentally substantiated. The initial stage of aggregation is generation of start aggregates. Each start aggregate contains hundreds of denatured protein molecules. The formation of start aggregates proceeds on the all-or-none principle. The intermediate states between the native enzyme forms and start aggregates were not detected. The further aggregation proceeds as a result of sticking of start aggregates and aggregates of higher order. For this stage of aggregation the diffusion-limited regime has been fulfilled. For this regime of aggregation each collision of the interacting particles results in their sticking. It was shown that the protective action of chaperones is due to the transition of the aggregation process into the kinetic regime, wherein the probability of sticking of the colliding particles becomes lower than unity.
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Unique equipment and/or methods:
    Instrument for measuring static and dynamic light scattering (PhotoCor Instruments, USA). Asymmetric flow field-flow fractionation (Wyatt Technology, USA). Refractometer Abbemat 500 (Anton Paar, Austria).
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Partners:
    – Academician A.M.Ostrovsky, Laboratory of physyco-chemical foundations of reception, Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, Russia.
    – Professor V.I.Muronets, Faculty of Bioengineering and Bioinformatics, Moscow State University, Moscow, Russia.
    – Dr. I.K.Yudin, Gas Research Institute, Russian Academy of Sciences, Moscow, Russia
    – Prof. Hai-Meng Zhou, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China.
    – Dr. Luciano Saso, Department of Pharmacology of Natural Substances and General Physiology, University of Rome "La Sapienza", Rome, Italy.
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Grants:
    INTAS, 93-2223, 1993-1997, the head B.I.Kurganov
    "Protein-protein interaction in microemulsions. From basic knowledge to applications"
    International Science Foundation, J9S100, 1995, the head B.I.Kurganov
    "Hysteretic properties of muscle glycogen phosphorylase b"
    Russian Foundation for Basic Research, 96-04-10016C (joint project with China Natural Science Foundation), 1997-1998, the head B.I.Kurganov
    "Denaturation and folding of enzymes"
    Russian Foundation for Basic Research, 99-04-48639, 1999-2001, the head B.I.Kurganov
    "Stability, denaturation, aggregation, and refolding of oligomeric enzymes"
    Russian Foundation for Basic Research, 99-04-48639, 2002-2004, the head B.I.Kurganov,
    "Protein aggregation. Mechanisms of protective action of chaperones and osmolytes"
    INTAS, 03-51-4813, 2004-2006, the head B.I.Kurganov (in collaboration with Laboratory of molecular organization of biological structures)
    "Mechanisms of protein denaturation and design of new inhibitors of protein aggregation for the pharmacological treatment of Alzheimer and other condensation diseases"
    Program "Molecular and Cell Biology"of Prezidium of Russian Academy of Sciences, 2003-2007, the head B.I.Kurganov (in collaboration with Laboratory of molecular organization of biological structures).
    "Denaturation and aggregation of proteins. Mechanisms of protective action of molecular chaperones"
    Russian Foundation for Basic Research 05-04-48691, 2005-2007, the head B.I.Kurganov
    "Investigation of mechanisms of protein aggregation and mechanisms of chaperone-like activity"
    Program "Molecular and Cell Biology"of Prezidium of Russian Academy of Sciences, 2008-2012, the head B.I.Kurganov
    "Protein aggregation and protective action of chaperones" (in collaboration with Laboratory of molecular organization of biological structures).
    Russian Foundation for Basic Research 08-04-00666-à, 2008-2010, the head B.I.Kurganov
    "Assessment of efficiency of agents suppressing protein aggregation".
    Russian Foundation for Basic Research 11-04-00932-à, 2011-2013, the head B.I.Kurganov
    "Mechanisms of suppression of protein aggregation by chaperones and the agents possessing the chaperone-like activity".
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Selected publications:
    1. Lyubarev, A.E., Kurganov, B.I. "Analysis of DSC data relating to proteins undergoing irreversible thermal denaturation". Journal of Thermal Analysis and Calorimetry, 2000, v.62, p.51-62.
    2. Kurganov, B.I., Kornilaev, B.A., Chebotareva, N.A., Malikov, V.P., Orlov, V.N., Lyubarev, A.E., Livanova, N.B. "Dissociative mechanism of thermal denaturation of rabbit skeletal muscle glycogen phosphorylase b". Biochemistry, 2000, v.39, No. 43, p.13144-13152.
    3. Luybarev, A.E., Kurganov, B.I. "Study of irreversible thermal denaturation of proteins by differential scanning calorimetry". Recent Research Developments in Biophysical Chemistry, 2001, v. 2, p.141-165.
    4. Wang, K., Kurganov, B.I. "Kinetics of heat- and acidification-induced aggregation of firefly luciferase", Biophysical Chemistry, 2003, v.106, p.97-109.
    5. Rafikova, E.R., Kurganov, B.I., Arutyunyan, A.M., Kust, S.V., Drachev, V.A., Dobrov, E.N. "A mechanism of macroscopic (amorphous) aggregation of the tobacco mosaic virus coat protein", International Journal of Biochemistry and Cell Biology, 2003, v.35, 1452–1460.
    6. Meng, F.-G., Hong, Y.-K., He, H.-W., Lyubarev, A.E., Kurganov, B.I., Yan, Y.-B., Zhou, H.-M. "Osmophobic effect of glycerol on irreversible thermal denaturation of rabbit creatine kinase", Biophysical Journal, 2004, v. 87, No. 4, 2247-2254.
    7. Arispe, N., Doh, M., Simakova, O., Kurganov, B., De Maio, A. "Hsc70 and Hsp70 interact with phosphatidylserine on the surface of PC12 cells resulting in a decrease of viability", FASEB Journal, 2004, v. 18, 1636-1645.
    8. Kurganov, B., Doh, M., Arispe, N. "Aggregation of liposomes induced by the toxic peptides Alzheimer’s A(beta)s, human amylin and prion (106–126): facilitation by membrane-bound GM1 ganglioside", Peptides, 2004, v. 25, p. 217–232.
    9. Chebotareva N.A., Andreeva I.E., Makeeva V.F., Livanova N.B., Kurganov B.I. "Effect of molecular crowding on self-association of phosphorylase kinase and its interaction with phosphorylase b and glycogen", Journal of Molecular Recognition, 2004, v. 17, p. 426–432.
    10. Chebotareva N.A., Kurganov B.I., Harding S.E., Winzor D.J. "Effect of osmolytes on the interaction of flavin adenine dinucleotide with muscle glycogen phosphorylase b", Biophysical Chemistry, 2005, v. 113, p. 61–66.
    11. Shalova I.N., Asryants R.A., Sholukh M.V., Saso L., Kurganov B.I., Muronetz V.I., Izumrudov V.A. "Interaction of polyanions with basic proteins, 2(a): influence of complexing polyanions on the thermo-aggregation of oligomeric enzymes", Macromolecular Bioscience, 2005, v. 5, No. 12, p. 1184-1192.
    12. Khanova H.A., Markossian K.A., Kurganov B.I., Samoilov A.M., Kleimenov S.Yu., Levitsky D.I., Yudin I.K., Timofeeva A.C., Muranov K.O., Ostrovsky M.A. "Mechanism of chaperone-like activity. Suppression of thermal aggregation of betaL-crystallin by alpha-crystallin", Biochemistry, 2005, v. 44, No. 47, p. 15480-15487.
    13. Kurganov B.I. "Protein aggregation kinetics" in: Chemical and Biological Kinetics. New Horizons. Vol. 2. Biological Kinetics (E.B. Burlakova, S.D. Varfolomeev, eds), Koninklijke Brill NV, Leiden, The Netherlands, 2005, p. 251-279. ISBN: 90 6764 4315.
    14. Markossian K.A., Khanova H.A., Kleimenov S.Yu., Levitsky D.I., Chebotareva N.A., Asryants R.A., Muronetz V.I., Saso L., Yudin I.K., Kurganov B.I. "Mechanism of thermal aggregation of rabbit muscle glyceraldehyde 3 phosphate dehydrogenase", Biochemistry, 2006, v. 45, p. 13375-13384.
    15. Panyukov Y.V., Nemykh M.A., Rafikova E.R., Kurganov B.I., Yaguzhinsky L.S., Arutyunyan A.M., Drachev V.A., Dobrov E.N. "Low cetyltrimethylammonium bromide concentrations induce reversible amorphous aggregation of tobacco mosaic virus and its coat protein at room temperature", International Journal of Biochemistry and Cell Biology, 2006; v. 38, No. 4, p. 533-543.
    16. Chebotareva N.A., Meremyanin A.V., Makeeva V.F., Kurganov B.I. "Self-association of phosphorylase kinase under molecular crowding conditions". Progress in Colloid and Polymer Science, 2006, v. 131, p. 83-92.
    17. Ospanov R., Kochetov G., Kurganov B. "Theoretical model of interactions between ligand-binding sites in a dimeric protein and its application for the analysis of thiamine diphosphate binding to yeast transketolase", Biophysical Chemistry, 2006, v. 124, p. 106–114.
    18. Markossian K., Kurganov B., Levitsky D., Khanova H., Chebotareva N., Samoilov A., Eronina T., Fedurkina N., Mitskevich L., Merem’yanin A., Kleymenov S., Makeeva V., Muronets V., Naletova I., Shalova I., Asryants R., Schmalhausen E., Saso L., Panyukov Yu., Dobrov E., Yudin I., Timofeeva A., Muranov K., Ostrovsky M. "Mechanisms of chaperone-like activity", in: Protein Folding: New Research (Obalinsky, T.R., Ed.) (Nova Science Publishers Inc, New York, 2006) p. 89-171. ISBN: 1-59454-835-8.
    19. Khanova H.A., Markossian K.A., Kleimenov S.Yu., Levitsky D.I., Chebotareva N.A., Golub N.V., Asryants R.A., Muronetz V.I., Saso L., Yudin I.K., Muranov K.O., Ostrovsky M.A., Kurganov B.I. "Effect of alpha-crystallin on thermal denaturation and aggregation of rabbit muscle glyceraldehyde-3-phosphate dehydrogenase", Biophysical Chemistry, 2007, v. 125, p. 521-531.
    20. Panyukov Y., Yudin I., Drachev V., Dobrov E., Kurganov B. "The study of amorphous aggregation of tobacco mosaic virus coat protein by dynamic light scattering", Biophysical Chemistry, 2007, v. 127, p. 9-18.
    21. Amani M., Moosavi-Movahedi A.A., Floris G., Mura A., Kurganov B.I., Ahmad F., Saboury A.A. "Two-state irreversible thermal denaturation of Euphorbia characias latex amine oxidase", Biophysical Chemistry, 2007, v. 125, p. 254-259.
    22. Golub N., Meremyanin A., Markossian K., Eronina T., Chebotareva N., Asryants R., Muronets V., Kurganov B. "Evidence for the formation of start aggregates as an initial stage of protein aggregation", FEBS Letters, 2007, v. 581, p. 4223-4227.
    23. Lyubarev A.E., Kurganov B.I. "Study of irreversible thermal denaturation of proteins by differential scanning calorimetry" in: Protein Structures, Methods in Protein Structures and Stability Analysis (V.N. Uversky, Eu.A. Permyakov, Eds) Nova Science Publishers, Inc., 2007, pp. 109-145. ISBN: 1-60021-704-4.
    24. Meremyanin A.V., Eronina T.B., Chebotareva N.A., Kurganov B.I. "Kinetics of thermal aggregation of glycogen phosphorylase b from rabbit skeletal muscle. Mechanism of protective action of alpha-crystallin”, Biopolymers, 2008, v. 88, No. 2, p. 124-134.
    25. Chebotareva N.A., Meremyanin A.V., Makeeva V.F., Livanova N.B., Kurganov B.I. "Cooperative self-association of phosphorylase kinase from rabbit skeletal muscle", Biophysical Chemistry, 2008, v. 133, No. 1-3, p. 45-53.
    26. Golub, N.V., Markossian, K.A., Kasilovich, N.V., Sholukh, M.V., Kurganov, B.I. "Thermal inactivation, denaturation and aggregation of mitochondrial aspartate aminotransferase", Biophysical Chemistry, 2008, v. 135, No. 1-3, p. 125-131.
    27. Markossian K.A., Golub N.V., Khanova H.A., Levitsky D.I., Poliansky N.B., Muranov K.O., Kurganov B.I. "Mechanism of thermal aggregation of yeast alcohol dehydrogenase I. Role of intramolecular chaperone", Biochimica et Biophysica Acta, 2008, v. 1784, No. 9, p. 1286-1293.
    28. Amani M., Yousefi R., Moosavi-Movahedi A.A., Pintus F., Mura A., Floris G., Kurganov B.I., Saboury A.A. "Structural changes and aggregation process of Cu/containing amine oxidase in the presence of 2,2,2'-trifluoroethanol", Protein & Peptide Letters, 2008, v. 15, No. 5, p. 521-527.
    29. Golub, N.V., Markossian, K.A., Sholukh, M.V., Muranov, K.O., Kurganov, B.I. "Study of kinetics of thermal aggregation of mitochondrial aspartate aminotransferase by dynamic light scattering: protective effect of alpha-crystallin", European Biophysics Journal, 2009, v. 38, No. 5, p. 547–556.
    30. Markossian, K.A., Golub, N.V., Kleymenov, S.Yu., Muranov, K.O., Sholukh, M.V., Kurganov, B.I. "Effect of alpha-crystallin on thermostability of mitochondrial aspartate aminotransferase", International Journal of Biological Macromolecules, 2009, v. 44, No. 5, p. 441–446.
    31. Markossian, K.A., Yudin, I.K., Kurganov, B.I. "Mechanism of suppression of protein aggregation by alpha-crystallin. Review", International Journal of Molecular Sciences, 2009, v. 10, No. 3, p. 1314-1345.
    32. Eronina, T.B., Chebotareva, N.A., Bazhina, S.G., Makeeva, V.F., Kleymenov, S.Y., Kurganov, B.I. "Effect of proline on thermal inactivation, denaturation and aggregation of glycogen phosphorylase b from rabbit skeletal muscle", Biophysical Chemistry, 2009, v. 141, No. 1, p. 66-74.
    33. Bumagina, Z.M., Gurvits, B.Ya., Artemova, N.V., Muranov, K.O., Kurganov, B.I. "Mechanism of suppression of dithiothreitol-induced aggregation of bovine alpha-lactalbumin by alpha-crystallin", Biophysical Chemistry, 2010, v. 146, No. 2-3, p. 108-117.
    34. Markossian, K.A., Golub, N.V., Chebotareva, N.A., Asryants, R.A., Naletova, I.N., Muronetz, V.I., Muranov, K.O., Kurganov, B.I. "Comparative analysis of the effects of alpha-crystallin and GroEL on the kinetics of thermal aggregation of rabbit muscle glyceraldehyde-3-phosphate dehydrogenase", The Protein Journal, 2010, v. 29, No. 1, p. 11-25.
    35. Maloletkina, O.I., Markossian, K.A., Belousova, L.V., Kleimenov, S.Y., Orlov, V.N., Makeeva, V.F., Kurganov, B.I. "Thermal stability and aggregation of creatine kinase from rabbit skeletal muscle. Effect of 2-hydroxypropyl-beta-cyclodextrin". Biophysical Chemistry, 2010, v.148, No. 1-3, p. 121–130.
    36. Maloletkina, O.I., Markossian, K.A., Asryants, R.A., Semenyuk, P.I., Makeeva, V.F., Kurganov, B.I. "Effect of 2-hydroxypropyl-beta-cyclodextrin on thermal inactivation, denaturation and aggregation of glyceraldehyde-3-phosphate dehydrogenase from rabbit skeletal muscle", International Journal of Biological Macromolecules, 2010, v. 46, p. 487–492.
    37. Eronina, T.B., Chebotareva, N.A., Bazhina, S.G., Kleymenov, S.Y., Naletova, I.N., Muronetz, V.I., Kurganov, B.I. "Effect of GroEL on thermal aggregation of glycogen phosphorylase b from rabbit skeletal muscle", Macromolecular Bioscience, 2010, v. 10, No. 7, p. 768-774.
    38. Chebotareva, N.A., Makeeva, V.F., Bazhina, S.G., Eronina, T.B., Gusev, N.B., Kurganov, B.I. "Interaction of Hsp27 with native phosphorylase kinase under crowding conditions", Macromolecular Bioscience, 2010, v. 10, No. 7, p. 783-789.
    39. Eronina, T.B., Chebotareva, N.A., Kleymenov, S.Y., Roman, S.G., Makeeva, V.F., Kurganov, B.I. "Effect of 2-hydroxypropyl-beta-cyclodextrin on thermal stability and aggregation of glycogen phosphorylase b from rabbit skeletal muscle". Biopolymers, 2010, v. 93, No. 11, p. 986–993.
    40. Markov, D.I., Zubov, E.O., Nikolaeva, O.P., Kurganov, B.I., Levitsky, D.I. "Thermal denaturation and aggregation of myosin subfragment 1 isoforms with different essential Light chains". International Journal of Molecular Sciences, 2010, v. 11, p. 4194-4226.
    41. Muranov K.O., Maloletkina O.I., Poliansky N.B., Markossian K.A., Kleymenov S.Yu., Rozhkov S.P., Goryunov A.S., Ostrovsky M.A., Kurganov B.I. "Mechanism of aggregation of UV-irradiated betaL-crystallin", Experimental Eye Research, 2011, v. 92, No. 1, pp. 76-86.
    42. Amani, M., Khodarahmi, R., Ghobadi, S., Mehrabi, M., Kurganov, B.I., Moosavi-Movahedi, A.A. "Differential scanning calorimetry study on thermal denaturation of human carbonic anhydrase II", Journal of Chemical & Engineering Data, 2011, v. 56, No. 4, p. 1158–1162.
    43. Eronina T., Borzova V., Maloletkina O., Kleymenov S., Asryants R., Markossian K., Kurganov B. "A protein aggregation based test for screening of the agents affecting thermostability of proteins", PLoS One, 2011, 6(7): e22154.
    44. Roman, S.G., Chebotareva, N.A., Eronina, T.B., Kleymenov, S.Yu., Makeeva, V.F., Muranov, K.O., Poliansky, N.B., Kurganov, B.I. "Does crowded cell-like environment reduce the chaperone-like activity of alpha-crystallin?" Biochemistry, 2011, v. 50, No. 49, p. 10607-10623.
    45. Bekasova O.D., Chebotareva N.A., Safenkova I.V., Rusanov A.L., Kurganov B.I. "Effect of CdS nanoparticles on the properties of a protein matrix", Inorganic Materials, 2011, Volume 47, No. 8, p. 830-836.
    46. Roman S.G., Chebotareva N.A., Kurganov B.I. "Concentration dependence of chaperone-like activities of alpha-crystallin, alphaB-crystallin and proline." International Journal of Biological Macromolecules, 2012, v. 50, No. 5, p. 1341-1345.
    47. Maloletkina O.I., Markossian K.A., Chebotareva N.A., Asryants R.A., Kleymenov S.Yu., Poliansky N.B., Muranov K.O., Makeeva V.F., Kurganov B.I. "Kinetics of aggregation of UV-irradiated glyceraldehyde-3-phosphate dehydrogenase from rabbit skeletal muscle. Effect of agents possessing chaperone-like activity", Biophysical Chemistry, 2012, v. 163-164, p. 11-20.
    48. Kurganov B.I. "Fundamental aspects of conformational lability of proteins." Biochemistry and Analytical Biochemistry, 2012, 1(4): e107.
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COMPUTER BIOCHEMISTRY GROUP
       The basic scientific theme of group is the bioinformatics of the natural oligopeptides and protein fragments, which contain from 2 up to 50 amino acid residues. With the help of computer methods the biochemical and biophysical problems of these substances are solved. The group is created in 1993 and to the present time consists of 4 persons.
       The head of group is doctor of biological sciences, candidate of physical and mathematical sciences, professor Alexander A. Zamyatnin, author more than 200 scientific publications devoted dilatometry of proteins, to the problem of muscle contraction, and computer biochemistry, member European Peptide Society since 1995.
       The group created a unique computer database EROP-Moscow (Endogenous Regulatory OligoPeptides), containing the data on structure, functions, and localization more than 10000 natural oligopeptides and being accessible in the Internet. On the basis of this base the common physicochemical features of different functional classes of oligopeptides, such as neuropeptides, peptide hormones and toxins, antimicrobial peptides, and also immunoregulators have been studied.
       Now the group develops new scientific direction FRAGMENTOMICS - system research of structure and functions of food protein fragments and their role in regulatory processes. Except for this, search for structures of new regulatory oligopeptides in plants is performed. In this research the data of the base EROP-Moscow and also unique computer programs created by the members of group are used.

    Grants:
    1. RFBR, 1994, "Computer study of structural-functional relationships of oligopeptide bioregulators", the head À.À.Zamyatnin.
    2. RFBR, 1997-1999, "Computer study of biochemical features of physiologically active oligopeptides", the head À.À.Zamyatnin.
    3. GCST "Biosensors", 1996, "Creation of the computer database of the natural oligopeptide ligands for biosensor devices", the head À.À.Zamyatnin.
    4. GCST "The modern methods of bioingeneering. Out-of-cell synthesis of protein", 1996-2000, "Computer study of physicochemical and biological features of antimicrobial oligopeptides", the head À.À.Zamyatnin.
    5. GCST "Creation of new drug – immunocorrector of dipeptide nature", 2000, the head À.À.Zamyatnin.
    6. RFBR "Creation of client-server EROP-Moscow knowledgebase for endogenous oligopeptides with Internet access", 2002-2005, the head À.À.Zamyatnin.
    7. Participation in the Program "Molecular and cellular biology" of the Russian Academy of Sciences, 2003-2007, the head B.I.Kurganov.
    8. FONDECYT (Chilean National Research Foundation) “Elucidation of the first regulatory oligopeptides in grape”, 2008-2009, the head À.À.Zamyatnin.

    Selected Publications:
    1. Zamyatnin A.A., Voronina O.L. “Computer biochemistry and molecular physiology of the natural peptide ligands: different functional groups”, Science - Present and Future (Y.Schimonishi, Ed.), Kluwer Academic Publishers, Dordrecht, 1999, p. 212-214, ISBN: 0-7923-5271-8.
    2. Zamyatnin A.A. “Computer biochemistry and molecular technology of the underwater biosensor creation: study of the natural chemical substance population”, Proceedings of the 2000 International Symposium on Underwater Technology, Tokyo (Japan), 2000, p. 451-456, ISBN: 0-7803-63-78-7.
    3. Zamyatnin A.A. “Database for underwater biosensor ligands”, Proceedings of the 2002 International Symposium on Underwater Technology, Tokyo (Japan), 2002, p. 331-335.
    4. Zamyatnin A.A., Borchikov A.S., Vladimirov M.G., Voronina O.L. “The EROP-Moscow oligopeptide database”, Nucleic Acids Research, 2006, v. 34, Database issue, p. D261-D266.
    5. Zamyatnin A.A. “Structural and functional diversity of endogenous regulatory oligopeptides”, Peptides 2006, Proceedings of the 29th European Peptidide Symposium (K.Rolka, P.Rekowski, and J.Silberring, Eds.), Geneva, Kenes International, 2007, p. 384-385, ISBN: 978-965-555-297-3.
    6. Zamyatnin A.A., Borchikov A.S. “Peptidomics of grape”, Journal of Peptide Science, 2008, v. 14, No 8, p. 128-128.
    7. Zamyatnin A.A., Borchikov A.S. “Protein-oligopeptide fragmentomics”, Journal of Peptide Science, 2008, v. 14, No 8, p. 164-164.
    8. Zamyatnin A.A. “Protein-oligopeptide fragmentomics”, Biophysics, 2008, v. 53, No 5, p. 329-335.
    9. Zamyatnin A.A., Borchikov A.S. “Peptidomics of grape”, Peptide 2008, Proceedings of the 30th European Peptide Symposium (H.Lankinen Ed.), Finnish Peptide Society and European Peptide Society, Finland, 2008, p. 356-357, ISBN: 978-952-92-5198-8.
    10. Zamyatnin A.A. “Protein-oligopeptide fragmentomics”, Peptide 2008, Proceedings of the 30th European Peptide Symposium (H.Lankinen Ed.), Finnish Peptide Society and European Peptide Society, Finland, 2006, p. 566-567, ISBN: 978-952-92-5198-8.
    11. Zamyatnin A.A. “Putative antimicrobial oligopeptides in grape Vitis vinifera”, International Journal of Health Science, 2009, v. 2, No 2, p. 179-183.
    12. Zamyatnin A.A. “Fragmentomics: A new insight intp structures and functions of the natural oligopeptide diversity”, Recent Advances in Biomedical Electronics and Biomedical Informatics (C.R.Long, P.Anninos, T.Pham, P.Anasstasopouos, and N.E.Mastorakis, Eds.), WSEAS Press, Russia (Moscow), 2009, p. 170-176, ISBN: 978-960-474-110-6.
    13. Zamyatnin A.A. “Fragmentomics of natural peptide structures”, Biochemistry (Moscow), 2009, 75, ¹ 13, p. 1575-1585.
    14. Zamyatnin A.A. “A new way of natural oligopeptide primary structure elucidation”, Recent Advances in Biology, Biophysics, Bioengineering and Computational Chemistry (C.A.Bulusea, V.Mladenov, E.Pop, M.Leba, and N.Mastorakis, Eds.), WSEAS Press, Spain (Puerto de la Cruz), 2009, p. 33-38, ISBN: 978-960-474-141-0.
    15. Zamyatnin A.A. “Peptide fragmentomics”, Journal of Peptide Science, 2010, v. 16, Suppl. 1, p. 37-37.
    16. Zamyatnin A.A., Voronina O.L. “Antimicrobial oligopeptides and rapid alkalinization factors in Chilean grape”, Journal of Peptide Science, 2010, v. 16, Suppl. 1, p. 150-151.
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