LABORATORY OF BIOCHEMISTRY AND PLANT IMMUNITY
Head of Laboratory:
Structure of Laboratory:
Group "Induced Resistance of Plants"
Head: Ozeretskovskaya Olga Leonidovna, D. Biol. Sci., professor, known specialist in the area of plant defence from disease and stresses.
Group of Regulation of Plant Dormancy and Resistance to Disease
Head: Nataliya P. Korableva, Ph. D., D. Biol. Sci., professor
e-mail: ozeretskovskaya AT inbi.ras.ru
e-mail: korableva AT inbi.ras.ru
The laboratory consists of 4 doctors of biological sciences, 5 candidates of biological sciences, 6 workers without scientific degrees.
The areas of interests:
The investigations are applied to the study of mechanisms of phytoimmunity, which was the basis for the plant resistance against biotic and abiotic stresses, the signal systems, determining the induction or inhibition of plant immune responses and also of the processes of wound reparation. The principle of the induction of systemic prolonged plant resistance with biogenic elicitors, evolved early by the researchers of laboratory, allows to increase of immune potential of plants and has made possible to increase the crop, the quality of the agricultural production and the more long storage period.
There is the screening of elicitors with systemic prolonged action is realized for increasing plant resistance against stresses and diseases. The components of pathogens and plants (polyenoic fat acids, fragments of chitin and chitosan. β-1,3-glucans, xyloglucans, and also composition of elicitors and signal molecules and their fragments are used as elicitors. The interplay of structure and function of immunomodulators, determinating the defense mechanisms of plants against biotic and abiotic stresses are investigated.
The investigation of process of plant tissues wound reparation, which is the one of more ancient defense reactions, arrests our attention. The rate and intensity of wound reparation are investigated on a basis of the estimation of forming of the second meristem - phellogen and defense properties of periderm, and this is essential for safety the crop of agricultural production.
Fundamental studies on biochemical and molecular mechanisms of the regulation of plant growth and resistance to biotic (plant pathogens) and abiotic (unfavorable temperature, water deficiency at al.) stresses are the major problems to be investigated. The investigations are based on concept about interrelations of ontogenetic stage and resistance in plants. As had been shown by long-standing investigations, the development of immune response is related to the length of deep dormancy in potato tuber of different variety that is determined genetically. The capacity of plant tissues to enhanced synthesis of therpenoids and phenolics (the hormone of dormancy – abscisic acid, constitutive and induced antibiotics) is one of the important constituents of defensive response.
A study of the signal systems of plant cells and their role in the development of resistance is the topical direction in the field of plant dormancy and immunity regulation. Studies of molecular mechanisms of the action of signal molecules (ethylene, abscisic and jasmonic acids, brassinosteroids at al.) includes the evaluation of functional activity of plasma membrane enzymes (of H+ATPase, Ca+ATPase), modification of cell ultrastructure, changes in stress phytohormone biosynthesis, variations of the role of polygalacturonase inhibiting protein (PGIP) in the resistance. The investigation of PGIP is of importance because it increases plant resistance to diseases by inhibition of the activity of polygalacturonase – main pathogenic factor of pathogen, and besides, is involved in the regulation of processes of plant cell growth and cell wall elongation at different ontogenetic stages.
The results of the investigations are the basis for further study of mechanisms of the interaction between different systems of signal transduction into plant cell, biochemical processes in plant related to the regulation of growth, dormancy, aging, and immunity. The using for the experiments of transgenic plants with inserted genes of taumatin, defensin et al. that are involved in the regulation of resistance allows to reveal post transformational changes in the reaction of plants on the effect of signal molecules and physiologically active substances.
The screening of the inducers of dormancy and resistance for development of ecologically harmless methods to increase the resistance of potato, vegetables and fruits during cultivation and storage of agricultural products.
|Last review: 19, October, 2010|
|© A.N.Bach Institute of Biochemistry of the R.A.S., 2001-2010|