LABORATORY OF CHEMICAL ENZYMOLOGY
Head of Laboratory:
Prof. A.I. Yaropolov,
He has great experience in bioelectrocatalysis, in studies of the mechanism of redox enzymes and free radical reactions, in development of heterogeneous catalysts based on immobilized enzymes, creation of various types of biosensors. He is one of the authors of the discovery called ďbioelectrocatalysisĒ of oxidoreductases (laccase, peroxidase, hydrogenase). He is the author of 8 patents and more that 130 publications in refereed journals
e-mail: yaropolov AT inbi.ras.ru
General description of the Laboratory:
The Laboratory of Chemical Enzymology was founded after reorganization of the Laboratory of Kinetic of Biochemical Processes.The Laboratory was originally focused on the chemical, structural and electrochemical investigations of the redox enzymes and proteins, as their application in various areas of biotechnology. The major problems facing the laboratory are bioelectrocatalysis; study of mechanisms of redox enzymes and free radical reactions; investigation of environmentally friendly technology of green organic synthesis; creation of various types of biosensors and biofuel cells. At present time the Laboratory comprises 10 staff researchers including young scientists.
Scientific interests, priorities:
- physico-chemical study of oxidoreductases and their application in biotechnology;
- investigation of the mechanism of free radical reactions during oxidoreductase function;
- delicate organic synthesis using oxidoreductases;
- bioelectrochemical studies of oxidoreductases, including direct (mediator-less) electron transfer and bioelectrocatalysis;
- biosensors and biofuel cells;
- biodegradation of xenobiotics: selection and investigation of redox mediators for the degradation of xenobiotics using laccase-mediator systems.
Main directions of the present activity:
- study of homogeneous and heterogeneous catalysis of multicopper oxidases including bioelectrocatalysis; possible application of fundamental knowledge for the creation of biosensors and biofuel cells.
- green synthesis of electroconductive polyaniline by oxidoreductases
Basic accomplished projects:
1. Studies of electrochemical properties of monoamino oxidase in reactions with artificial acceptors of electrons.
2. Investigation of free radical reactions in laccase-mediator systems under enzymatic degradation of xenobiotics and pulp biobleaching.
3. Development and study of mechanism of environmentally friendly synthesis of water-soluble electroconductive polyaniline by laccase and peroxidase.
1. Possible application of laccases in green organic synthesis
2. Novel biosensors and analysis kits based on genetically engineered biomolecules for formaldehyde assay
Main scientific progress:
1. Phenomena of bioelectrocatalysis by oxidoreductases was discovered and several mechanisms of enzymeís functioning on different electrodes were suggested.
2. New class of redox mediators (enhancers) of oxidoreductases was found, phenyl-methyl-pyrazolones, which could employed in the processes of the degradation of xenobiotics and pulp and paper industries for chlorine-free bleaching.
3. Biosensors for the determination of lignin and different phenolic compounds, including chlorine-containing substituted aromatics substances, were designed.
4. New artificial electron acceptors of monoamino oxidase were found.
5. Environmentally friendly methods of enzymatic organic synthesis of a conductive polyaniline based on different oxidoreductases were developed. Suspensions of nanoparticles of polyaniline were received based on the matrix of soluble linear sulphopolymers (matrix synthesis). New materials based on electroconductive polyaniline were received for corrosion and antistatic protection.
Unique equipments and methods:
1. An original enzymatic method of synthesis of electroconductive polyaniline
2. Macro- and micro-spectroelectrochemical cells for electrochemical studies of redox proteins and enzymes.
3. A method of the redox titration of complex redox proteins.
National and International Collaborations:
1. Prof. Sergey O. Bachurin; Department of Biological and Medical Chemistry, Institute of Physiologically Active Compounds RAS (Russia).
2. Prof. Sergey D. Varfolomeev; Department of Chemical Enzymology, Faculty of Chemistry, M.V. Lomonosov Moscow State University (Russia).
3. Dr. Sci. Ilya N. Kurochkin; Faculty of Chemistry and Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University (Russia).
4. Prof. Valentin V. Biryukov; Department of Ecological Biotechnology, Moscow State University of Enviromental Engineering (Russia).
5. Prof. Mikhail R. Tarasevich; Department of Inorganic Electrocatalysts, A.N. Frumkin Institute of Physical Chemistry and Electrochemistry, RAS (Russia).
6. Prof. Myhaylo V. Gonchar; Department of Genetics and Biotechnology, Institute of Cell Biology, NAS (Ukraine).
7. Dr. Donal Leech; Department of Chemistry, National University of Ireland, Galway (Ireland).
8. Prof. Renata Bilewicz; Faculty of Chemistry, University of Warsaw, Warsaw (Poland).
9. Prof. Lo Gorton; Department of Analytical Chemistry, Lund University (Sweden).
Participation in projects, grants, and programs:
1. Russian Foundation for Basic Research Grant Ļ 98-03-32144
ęElectrochemical properties of monoamino oxidases in reactions with artificial acceptors of electronsĽ, 1998-2000.
2. INCO Copernicus Ļ ICA2-CT-2000-10050
ęPulp biodegradation using lignolytic enzyme reactionsĽ, 2000-2003.
3. FNTP of Russian Ministry of Science and Technology ęBiocatalytic technologiesĽ Ļ 43.073.1.1.2505
ęPulp biodegradation using laccase-mediator systemsĽ, 2000-2003.
4. Russian Foundation for Basic Research Grant Ļ 02-04-48885 ęDevelopment and study of mechanism of environmentally friendly synthesis of water-soluble conducting polyaniline by laccasesĽ, 2002-2004.
5. Russian Foundation for Basic Research Grant Ļ 03-04-48937
ęFree radical reactions in the laccase-mediator system under enzymatic degradation of xenobioticsĽ, 2003-2005.
6. FNTP of Russian Ministry of Science and Technology Ļ 02.467.11.3004
ęDevelopment of industrial enzyme-based preparations for pulp and paper industry, textile industry, and bonding agents, and biologically-compatible plasticsĽ from March 30, 2005.
7. INTAS Ļ 03-51-6278
ęNovel biosensors and analysis kits based on genetically engineered biomolecules for formaldehyde assayĽ, 2004-2006.
|Last review: 17 June, 2006|
|© A.N.Bach Institute of Biochemistry of the R.A.S., 2001-2007|